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NeoGenomics
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Oxford Nanopore
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JBS Science
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GenScript corporation
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Nextera AS
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NuProbe
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CapitalBio Corporation
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Image Search Results
Journal: medRxiv
Article Title: Detection and characterization of Hepatitis B virus double-stranded linear DNA-derived covalently closed circular DNA in chronic hepatitis B patients
doi: 10.1101/2025.01.20.25320854
Figure Lengend Snippet: Tissue DNA from 56 patients was subjected to HBV-targeted NGS with unique molecular index (UMI) incorporated. HBV reads were extracted by mapping reads to HBV reference genomes and the consensus sequences were generated for each UMI family. HBV reads were analyzed for INDELs in the DR2-1 region. Samples containing more than 5 different deletions and each deletion has less than 5 supporting reads were considered “dsl-cccDNA positive” by HBV-targeted NGS assay. Total of 32 samples, including 30 positives, 2 negatives for dsl-cccDNA by HBV-targeted NGS assay were selected and PCR products from PSAD-cccDNA assays were subject to PSAD-cccDNA PCR-NGS with UMI for dsl-cccDNA study. HBV reads from PSAD-UMI cccDNA PCR-NGS were analyzed for INDELs in the DR2-1 region. INDELs that were concordant between the two independent NGS assays were identified as dsl-cccDNA and were further characterized.
Article Snippet: Library DNA was subjected to the JBS HBV-targeted
Techniques: Generated
Journal: medRxiv
Article Title: Detection and characterization of Hepatitis B virus double-stranded linear DNA-derived covalently closed circular DNA in chronic hepatitis B patients
doi: 10.1101/2025.01.20.25320854
Figure Lengend Snippet: Positions and lengths of the deletions are indicated with detection count and percentage of the total deletions detected in the region of nt 1600–1840 by both HBV-targeted and cccDNA PCR-NGS assays.
Article Snippet: Library DNA was subjected to the JBS HBV-targeted
Techniques: